![]() The annotation will be drawn directly onto the image. Select Analyze › Microscope Measurement Tools › Draw Annotation - Line. Draw a "Straight Line" along the feature you want to measure (the length is displayed in the Fiji status bar)Ģ. Run Analyze › Microscope Measurement Tools › Choose Microscope Calibration - the pop-up window shows the new names and calibration values you set in your user-settings file.Īnnotating your image with a length measurementġ. You should only have to do this calibration once, when you do your initial calibration/setup.Īpplying a pre-configured scale to your imageġ1. It should have picked up the new settings now. Delete the " py.class" file in the plugin’s folder:įiji.app / plugins / Scripts / Analyze / Microscope Measurement Tools / Microscope_Calibrations_user_settings$py.classġ0. Unfortunately, changes to the settings file may not be automatically picked up by Fiji.ĩ. functions like myList.append( ) or myList = * 5 etc. This settings file is an actual python script, so you can edit the lists there using normal Python syntax. Type in the Calibration's Names & Scale values, making sure to match up the list of names with their corresponding calibrations. Perform more calibrations (on other images) if desired, recording the Scale values for each.įiji.app / plugins / Scripts / Analyze / Microscope Measurement Tools / Microscope_Calibrations_user_settings.pyħ. Type in the known length in " Known Distance" and record the " Scale" value for this calibration. Select the Analyze › Set Scale menu item.ĥ. Draw a line along the feature of known length.Ĥ. Open an image with a feature of some known length, taken with some microscope/objective that you desire to save a calibration for.ģ. zip file linked at GitHub/Releases, and place the resulting Analysis folder insideįiji.app / Plugins / Scripts (If an Analysis folder already exists, do Not overwrite it - you must have another Analysis plugin installed! Instead, merge the two folders.)Ģ. Please see the complete installation & usage instructions here: Settings file that contains your pre-configured scale calibrations, along with some settings for drawing annotations (background/text color etc.).Microscope_Calibrations_user_settings.py.Converts a Line ROI into a drawn annotation with the measurement length indicated.Opens the "Choose Calibration" window, for setting the measurement scale to a preconfigured value.Together, these results demonstrate that AGPG can drive endocrine therapy resistance and indicate that it is a promising biomarker and potential therapeutic target in breast cancer.Error creating thumbnail: Unable to save thumbnail to destination Coadministration of chemically modified AGPG siRNA and tamoxifen strongly suppressed tumor growth in endocrine-resistant cell line–derived xenografts. In patients with breast cancer, E2F1 target genes were positively and negatively correlated with expression of AGPG and PURα, respectively. Mechanistically, AGPG physically interacted with PURα, thus releasing E2F1 from PURα and leading to E2F1 signaling activation in ERα+ breast cancer cells. The upregulation of AGPG mediated resistance to endocrine therapy and cyclin-dependent kinase 4/6 inhibition in breast cancer cells. AGPG was also abnormally upregulated in patient breast tumors, especially in the luminal B subtype, and high AGPG expression was associated with poor survival of patients with ERα+ breast cancer receiving endocrine therapy. Expression of AGPG was increased in endocrine-resistant breast cancer cells, which was driven by epigenomic activation of an enhancer. Here, we identified the long noncoding RNA (lncRNA) actin gamma 1 pseudogene 25 (AGPG) as a regulator of E2F1 activity in endocrine-resistant breast cancer. A better understanding of the mechanisms governing E2F1 activity in resistant cells could reveal strategies for overcoming resistance. Endocrine therapy resistance is commonly mediated by activated E2F signaling. Resistance to endocrine therapy represents a major concern for patients with estrogen receptor α–positive (ERα+) breast cancer. ![]()
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